溶解性 | solubility(H2O): soluble 1 mg/mL, clear, colorless |
存貯條件 | 儲存溫度-20°C |
密度 | 1.1000 |
應用 |
分離DNA和RNA期間使核苷酸酶蛋白失活。 去除內毒素對陽離子蛋白的約束,如溶菌酶和核糖核酸酶。 研究報告顯示用于分離肝,酵母,綠豆線粒體 測定細胞膜上的酶 用于抗體標記,石蠟包埋組織切片中暴露抗原結合位點。 消化腦組織樣本中的蛋白質,用于朊病毒的傳染性海綿狀腦病(TSE)的研究 |
產品介紹 | 蛋白酶K是一個穩定,高活性絲氨酸蛋白酶。從晶體和分子結構研究的證據表明這種酶屬于枯草桿菌家族,帶有活性催化位點催化三鍵(Asp39,His69 - Ser224)。它是在寬的的環境范圍內保持穩定:pH值,緩沖鹽,去垢劑(SDS),和溫度。在0.1-0.5% SDS存在下,在分離DNA中,蛋白酶K保持活性,消化各種蛋白質和核酸酶。 |
備注 |
Unit Definition One unit will hydrolyze urea-denatured hemoglobin to produce colequivalent to 1.0 μmole of tyrosine per min at pH 7.5 at 37 °C (colby Folin-Ciocalteu reagent). |
生化機理 | Proteinase K is a stable highly reactive serine protease. Evidence from crystal molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp39-His69-Ser224). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity will digest a variety of proteins nucleases in DNA preparations without compromising the integrity of the isolated DNA. |
別名 | 蛋白酶K;Proteinase KEndopeptidase K Prok Protease K Tritirachium album proteinase K |